Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10560073 | Talanta | 2005 | 8 Pages |
Abstract
A rapid and sensitive method for the determination of alkylating agents in complex reaction mixtures was developed and characterized. Analyses are based on the alkylation of 2-mercaptopyridine by the analyte; the derivative is separated by RP-HPLC and measured by fluorescence detection. When applied to the determination of dimethyl sulfate, the method is linear over four orders of magnitude: 0.01-10 μg mLâ1. By using recrystallized 2-mercaptopyridine, quantitation limits of 10 ng mLâ1 can be achieved. Precision of the assay is 2% R.S.D. in the 1-10 μg mLâ1 range and about 15% R.S.D. at 10 ng mLâ1. Studies on the pH dependence of the derivatization reaction were key to minimizing interference from the dimethyl sulfate degradation product, monomethyl sulfate, in quenched reaction samples.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
J.G. Hoogerheide, R.A. Scott,