Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10560093 | Talanta | 2005 | 9 Pages |
Abstract
The immunosensor devices were based on a specific reaction of rabbit IgG with its biotinylated antibodies, which were immobilised on the modified screen-printed carbon electrodes through the streptavidin:biotin reaction. The immunosensors were used for a direct determination of AP labelled rabbit IgG, and for free rabbit IgG detection using a sequential competitive immunoassay. A calibration curve in the range of 5 à 10â11 to 1 à 10â9 M of rabbit IgG was obtained with a estimated detection limit of 5 à 10â11 M (7.0 ng/ml). These immunosensors were stable for 5 months if they were stored at 4 °C.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
MarÃa DÃaz-González, David Hernández-Santos, MarÃa Begoña González-GarcÃa, AgustÃn Costa-GarcÃa,