Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10560131 | Talanta | 2011 | 6 Pages |
Abstract
A new electrochemiluminescence (ECL) detector for capillary electrophoresis (CE) based on tris(2,2â²-bipyridyl)ruthenium(II) (Ru(bpy)32+) immobilized in Nafion/PTC-NH2 (an ammonolysis product of 3,4,9,10-perylenetetracarboxylic dianhydride (PTCDA)) composite film was presented for the first time. The Nafion/PTC-NH2 composite film could effectively immobilize tris(2,2â²-bipyridyl)ruthenium(II) via ion-exchange and electrostatic interaction. Cyclic voltammetric and ECL behavior of Nafion/PTC-NH2/Ru composite film was investigated compared to Nafion/Ru composite. The Nafion/PTC-NH2/Ru composite film exhibited good ECL stability and simple operability. Then the CE with solid-state ECL detector system was successfully used to detect sophora - a quinolizidine type - alkaloids as sophoridine (SR) and matrine (MT). The CE-ECL parameters that affected separation and detection were optimized. Under the optimized conditions, the linear range was from 2.5Â ÃÂ 10â8 to 2Â ÃÂ 10â6Â mol/L for SR, 1.0Â ÃÂ 10â8 to 1.0Â ÃÂ 10â6Â mol/L for MT. The detection limit (S/NÂ =Â 3) was estimated to be 5Â ÃÂ 10â9 and 10â9Â mol/L for SR and MT, respectively. It was shown that the CE coupling with solid-state ECL detector system exhibited satisfying sensitivity of analysis.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Huijing Liu, Ruo Yuan, Yaqing Chai, Li Mao, Xia Yang, Ying Zhuo, Yali Yuan,