Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10560182 | Talanta | 2005 | 6 Pages |
Abstract
A simple method for the study of the pharmacokinetics of salbutamol using ion chromatography with direct conductivity detection without chemical suppression is presented in this paper. Baseline separation of salbutamol from plasma components was achieved by using diluted HNO3 (2 mmol lâ1) as mobile phase with acetonitrile (6%, v/v) as modifier. Atenolol was utilized as internal standard. Under the optimal conditions, the linear regression coefficients of the calibration curves are 0.996 within the concentration range of 1000-3 ng mlâ1 salbutamol. The detection limit (signal-to-noise ratio = 3) was 1 ng mlâ1 salbutamol. The noncompartmental pharmacokinetic parameters for salbutamol following administration of 8 mg salbutamol to 18 subjects were tested. The results coincided most satisfactorily with the results obtained by using reversed-phase high performance liquid chromatography (HPLC) with fluorescence detection.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jin Ouyang, Jing Li Duan, Willy R.G. Baeyens, Joris R. Delanghe,