Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10560221 | Talanta | 2005 | 5 Pages |
Abstract
It is found that the fluorescence of Tb-adenosine triphosphate (ATP)-phenanthroline (phen) system can be enhanced by Gd3+. The fluorescence enhancement of the Tb-Gd-ATP-phen system is considered to originate from intramolecular and intermolecular energy transfers, and the energy-insulating sheath effect of Gd-ATP-phen complex. In addition, a new energy transfer pathway in Tb-ATP-phen system is proposed. As a mediator, phen can transfer the energy absorbed by ATP to Tb3+ through the stacking action between aromatic ring of phen and purine ring of ATP. The proposed method has been used to determine trace amount of ATP. The detection limit is 5.4 Ã 10â9Â mol/l, which is about 40 times lower than that of the Tb-ATP-phen system. The proposed method is one of the most sensitive fluoremetries of ATP.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lei Li, Jinghe Yang, Xia Wu, Changxia Sun, Yang Liu, Shufang Liu, Benyu Su,