Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10560515 | Talanta | 2011 | 6 Pages |
Abstract
A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm Ã 50 mm + 3.0 mm Ã 100 mm and 4.6 Ã 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice.
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Authors
Lenka Krcmova, Dagmar Solichova, Bohuslav Melichar, Marketa Kasparova, Jiri Plisek, Luboš Sobotka, Petr Solich,