Article ID Journal Published Year Pages File Type
10560520 Talanta 2011 6 Pages PDF
Abstract
Pesticides may contaminate ground and surface waters and one of the major factors governing this property is soil sorption. Sorption can be assessed by batch equilibrium technique which produces lots of extracts with high dissolved organic carbon concentration in which the pesticide concentration has to be determined. We developed an ELISA procedure to analyse atrazine based on polyclonal antibodies (C193) for which tracer structure and dilutions of immunochemical reagents were adapted to fit the purpose. After a 1000-fold dilution (or after an SPE clean-up procedure) extracts of a sewage-sludge amended luvisol (used as an example application of the methodology developed) could be reliably analysed. The Freundlich model is able to describe adsorption for this system (r2 = 0.977) delivering a distribution coefficient KF of 1.6 ± 0.2 (mg kg−1) (mg L−1)−N and an isotherm nonlinearity factor N of 0.70 ± 0.09.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
, , , ,