Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10560608 | Talanta | 2005 | 7 Pages |
Abstract
The separate developments of microarray patterning of DNA oligonucleotides, and of DNA hairpins as sensitive probes for oligonucleotide identification in solution, have had a tremendous impact on basic biological research and clinical applications. Herein, we will discuss several successful efforts to develop oligonucleotide sensors based on the surface immobilization of functionalized DNA hairpins. We also will discuss the development of prototypical single-spot multianalyte “Molecular Beacon” biosensors. Importantly, we show that organic fluorophores will likely be inadequate in moving this technology forward and new approaches, such as the use of nanotechnology, will be needed.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Christopher M. Strohsahl, Hui Du, Benjamin L. Miller, Todd D. Krauss,