Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10560635 | Talanta | 2005 | 5 Pages |
Abstract
The oxidation of selected clinically important neurotransmitter metabolites with acidic potassium permanganate in the presence of polyphosphates evokes chemiluminescence of sufficient intensity to enable the sensitive determination of these species. Limits of detection for 5-hydroxyindole-3-acetic acid (5-HIAA), vanilmandelic acid (VMA; α,4-dihydroxy-3-methoxybenzeneacetic acid), 4-hydroxy-3-methoxyphenylglycol (MHPG), homovanillic acid (HVA, 4-hydroxy-3-methoxyphenylacetic acid) and 3,4-dihydroxyphenylacetic acid (DOPAC) were between 5 Ã 10â9 and 4 Ã 10â8 M, using flow-injection analysis methodology. In addition, we demonstrate the rapid determination of homovanillic acid and 5-hydroxyindole-3-acetic acid in human urine - without the need for extraction procedures - using monolithic column chromatography with chemiluminescence detection.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Jacqui L. Adcock, Neil W. Barnett, Jason W. Costin, Paul S. Francis, Simon W. Lewis,