Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10560639 | Talanta | 2005 | 7 Pages |
Abstract
This work reports a hybrid flow-injection analysis (FIA)/sequential-injection analysis (SIA) method for the rapid enzymatic assay of glucose with soluble glucose oxidase (GOD). The method relies on the sequential injection of segments of the sample and of a solution of enzyme by means of a multi-port selection valve in a flowing water stream. As the two zones are swept downstream, they overlap and merge so that the glucose in the sample is enzymatically oxidised. The generated hydrogen peroxide is merged with an alkaline luminol solution and the chemiluminescence (CL) intensity is monitored and related to the glucose concentration in the sample. The linear range of the method for glucose determination is 0.01-1 mmol Lâ1, the relative standard deviation is 3.9% at the 0.08 mmol Lâ1 level (n = 8), the limit of detection at the 2Ï level is 4 μmol Lâ1 glucose and the injection rate is 80 hâ1. The method was applied to the analysis of energy drinks and honey with relative errors in glucose determination in the range 100 ± 4.3%. The advantages of the proposed method are the wide linear range, the simple instrumentation used, the low consumption of sample and reagents, the elimination of catalysts and immobilised enzymes and the high sample throughput.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Polyxeni Panoutsou, Anastasios Economou,