Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10560808 | Talanta | 2005 | 5 Pages |
Abstract
A simultaneous determination of sulfamonomethoxine, sulfadimethoxine, and their hydroxy/N4-acetyl metabolites in chicken plasma, muscle, liver, and eggs using gradient high-performance liquid chromatography (HPLC) with a photo-diode array detector is developed. All the compounds are extracted by a handheld ultrasonic homogenizer with ethanol followed by centrifugation. The separation is performed by a reversed-phase C4 column with a gradient elution (ethanol:1% (v/v) acetic acid, v/v; 10:90 â 20:80). Average recoveries from samples spiked at 0.1-1.0 μg gâ1 or μg mlâ1 for each drug were >90% with relative standard deviations within 4%. The limits of quantitation were <30 ng gâ1 or ng mlâ1.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Kunihiro Kishida, Naoto Furusawa,