Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10560940 | Talanta | 2005 | 4 Pages |
Abstract
A novel fluorescence detector based on collinear scheme using a brightness light-emitting diode emitting at 470Â nm as excitation source is described. The detector is assembled by all-solid-state optical-electronic components and coupled with capillary electrophoresis using on-column detection mode. Fluorescein isothiocyanate (FITC) and FITC-labeled amino acids and small molecule peptide as test analyte were used to evaluate the detector. The concentration limit of detection for FITC-labeled phenylalanine was 10Â nM at a signal-to-noise ratio (S/N) of 3. The system exhibited good linear responses in the range of 1Â ÃÂ 10â7 to 2Â ÃÂ 10â5Â M (R2Â =Â 0.999).
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bingcheng Yang, Feng Tan, Yafeng Guan,