Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10561486 | Talanta | 2005 | 6 Pages |
Abstract
An HPLC-ECD method is described for the indirect determination of the hydroxyl (OH) radical. Fenton's reaction is used to produce OH, which simultaneously attacks phenols (phenol or pyrocatechol) to form the adducts, pyrocatechol or pyrogallic acid. Thus, [OH] quantification is based on the separation and detection of pyrogallic acid and/or pyrocatechol by an isocratic HPLC-ECD method. The quantification of OH is also performed alternatively by a chronoamperometric detection in an electrochemical cell, where simultaneously formed FeIII (Fenton's reaction) combines [FeII(CN)6]4â to produce the Prussian blue (PB) molecules (Fe4III[FeII(CN)6]3). Newly formed PB molecules are then immediately converted to colorless Everitts salt (K4Fe4II[FeII(CN)6]3) with the reduction of the high-spin FeIII to FeII at the surface of a glassy carbon electrode at +0.150Â V (versus Ag/AgCl). The calculated concentration of OH during incubation (0.626Â ppm) can be detected with negative errors by the HPLC-ECD (0.595 and 0.615Â ppm with the errors â5.2 and â1.8%, respectively) and by the chronoamperometric method (0.552 and 0.607Â ppm with the errors â11.8 and â3.0%, respectively). For the comparison of the two sets of data, HPLC-ECD method is much more promising.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Emrah Kilinc,