Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10561573 | Talanta | 2005 | 9 Pages |
Abstract
A solid-liquid extraction procedure followed by reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with a photodiode array detector (DAD) for the determination of polyphenols in freeze-dried apple peel and pulp is reported. The extraction step consists in sonicating 0.5Â g of freeze-dried apple tissue with 30Â mL of methanol-water-acetic acid (30:69:1, v/v/v) containing 2Â g of ascorbic acid/L, for 10Â min in an ultrasonic bath. The whole method was validated, concluding that it is a robust method that presents high extraction efficiencies (peel: >91%, pulp: >95%) and appropriate precisions (within day: R.S.D. (n = 5) <5%, and between days: R.S.D. (n = 5) <7%) at the different concentration levels of polyphenols that can be found in apple samples. The method was compared with one previously published, consisting in a pressurized liquid extraction (PLE) followed by RP-HPLC-DAD determination. The advantages and disadvantages of both methods are discussed.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Rosa M. Alonso-Salces, Alejandro Barranco, Edurne Corta, Luis A. Berrueta, Blanca Gallo, Francisca Vicente,