Article ID Journal Published Year Pages File Type
10562903 Talanta 2005 6 Pages PDF
Abstract
We developed a novel copolymer modified amperometric immunosensor for the detection of cholera antitoxin (anti-CT), by the electropolymerization of pyrrole-biotin and pyrrole-lactitobionamide monomers on platinum or glassy carbon electrodes. In the detection of cholera antitoxin we have used three enzymatic marker detection systems based on HRP-labeled rabbit IgG antibodies, biotinylated polyphenol-oxidase (PPO-B) and biotinylated glucose-oxidase (GOX-B). The comparison of the electro-enzymatic performances of these three configurations with different substrates, clearly shows that the more sensitive amperometric immunosensor was based on HRP with a lower limit of detection of 50 ng/ml anti-CT using hydroquinone/H2O2 system. The response time for this substrate was in range of 5-30 s. The HRP-amperometric immunosensor has thus proven to be a very sensitive tool to monitor nanomolar concentrations of anti-CT.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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