Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10564469 | Vibrational Spectroscopy | 2005 | 12 Pages |
Abstract
Aqueous solutions of l-histidine have been analysed in parallel by infrared (IR) and Raman spectroscopy over the pH range 0-14 with increments of one pH unit. The vibrational spectra in the region 2000-500Â cmâ1 have been interpreted and band positions have been assigned tentatively, taking into account assignments from literature after critical evaluation. As a result, a complete and complementary set of vibrational data has been obtained that can be used to determine all possible states of protonation of histidine, i.e. H4His2+, H3His+, H2His0, HHisâ and His2â. In addition, IR and Raman bands have been proposed as markers for the presence of the imidazole NÏ- or NÏ-protonated tautomeric forms of H2His0 and HHisâ.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
J. Gerbrand Mesu, Tom Visser, Fouad Soulimani, Bert M. Weckhuysen,