Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10564915 | Current Opinion in Chemical Biology | 2013 | 11 Pages |
Abstract
⺠Ammonia lyases and aminomutases are emerging in green synthetic routes to chiral amines. ⺠An ammonia lyase is being explored as an enzyme therapeutic for treating phenylketonuria and cancer. ⺠The restricted substrate range of the wild-type enzymes limits their widespread application. ⺠The non-reliance on external cofactors and direct functionalization of an olefinic bond make ammonia lyases attractive biocatalysts for use in the synthesis of natural and non-natural amino acids. ⺠The approach of combining structure-guided enzyme engineering with efficient mutant library screening has broadened the synthetic scope of these biocatalysts.
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Authors
Matthew M Heberling, Bian Wu, Sebastian Bartsch, Dick B Janssen,