| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10565238 | Current Opinion in Chemical Biology | 2005 | 7 Pages |
Abstract
The availability of vast gene repertoires from both natural sources (genomic and cDNA libraries) and artificial sources (gene libraries) demands the development and application of novel technologies that enable the screening or selection of large libraries for a variety of enzymatic activities. We describe recent developments in the selection of enzyme-coding genes for directed evolution and functional genomics. We focus on HTS approaches that enable selection from large libraries (>106 gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular.
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Amir Aharoni, Andrew D Griffiths, Dan S Tawfik,