Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10573984 | Journal of Inorganic Biochemistry | 2005 | 10 Pages |
Abstract
The formation of binary Ni(II) complexes with 2â²-deoxyguanosine 5â²-triphosphate (dGTP1, L) as well as ternary complexes thereof with l-histidine (His, A) was studied with the use of potentiometry and electronic absorption spectroscopy. In the binary and ternary systems, the complexes with stoichiometries NiH2Lâ, NiHL2â, NiL3â and NiH2LA2â, NiHLA3â, NiLA4â respectively, were detected. The ternary complexes are very stable at pH 7.4 and thus may constitute biologically relevant Ni(II) carriers in the cell. In the presence of hydrogen peroxide, the binary and ternary systems both generate hydroxyl radical-like species and undergo dGTP degradation with the formation of the 8-oxo-dGTP intermediate. The latter, along with dGTP complexation and degradation, may lead to mutagenesis and carcinogenesis due to base-mispairing properties of 8-oxoguanine and the disturbance in the physiological balance among the four canonical triphosphodeoxynucleotide substrates for DNA synthesis.
Related Topics
Physical Sciences and Engineering
Chemistry
Inorganic Chemistry
Authors
Piotr Kaczmarek, MaÅgorzata Jeżowska-Bojczuk, Wojciech Bal, Kazimierz S. Kasprzak,