Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10574216 | Journal of Inorganic Biochemistry | 2006 | 8 Pages |
Abstract
The effects exerted by the new complex cis-dichloro-1,2-propylenediaminetetraacetato ruthenium (III), H[RuCl2(PDTA-H2)] [1, RAP], on DNA and cultured tumor cells (ovarian carcinoma TG cell line) were studied. The comparative study of circular dichroism (CD) spectra obtained from DNA and RAP-DNA system evidences the interaction of the complex with DNA. Compound 1 also interacted with tumor TG cells to slow their proliferation rate. BrdU incorporation was enhanced in cells treated with compound 1, as evidenced by a single-cell electrophoresis method (comet assay), in accordance with RAP-induced DNA damage. DNA migration of compound 1-treated cells was similar to that induced by noxious agents other than cross-linking chemicals. The stability of [RuCl2(PDTA-H2)]-DNA binding is suggested by the high degree of damage that persisted after removal of compound 1 from the culture medium.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Inorganic Chemistry
Authors
Rosario A. Vilaplana, Fátima Delmani, Consolación Manteca, José Torreblanca, Javier Moreno, Gregorio GarcÃa-Herdugo, Francisco González-VÃlchez,