Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10582836 | Bioorganic Chemistry | 2005 | 7 Pages |
Abstract
Saccharomyces cerevisiae Thi20 is a fusion protein with homology to Bacillus subtilis ThiD and TenA. The N-terminus of Thi20 has significant sequence homology to B. subtilis ThiD, while the C-terminus has homology to B. subtilis TenA. Incubation of Thi20 with thiamin reveals that it has thiaminase II activity, in addition, incubation of Thi20 with HMP (4-amino-2-methyl-5-hydroxymethylpyrimidine) and ATP reveals that it has HMP kinase and HMP-P (4-amino-2-methyl-5-hydroxymethylpyrimidine phosphate) kinase activity. This demonstrates that Thi20 is a trifunctional protein with thiamin biosynthetic and degradative activity.
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Related Topics
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Organic Chemistry
Authors
Amy L. Haas, Nathan P. Laun, Tadhg P. Begley,