GC-MS detection and quantification of lipopolysaccharides in polysaccharides through 3-O-acetyl fatty acid methyl esters

Article ID	Journal	Published Year	Pages	File Type
10597900	Carbohydrate Polymers	2012	5 Pages	PDF

Abstract

âº We developed a GC-MS assay that could detect LPS through its 3-OH fatty acids. âº We could successfully detected sub ng LPS amounts on polysaccharides samples. âº Using a standard curve we could measure the LPS amount on various polysaccharides.

Keywords

LPS IL-1 Limulus Amebocyte Lysate LOQ LAL GC–MS SiE KDO β-glucan 3-Hydroxy fatty acids interleukin-1 tumor necrosis factor-alpha FAME TNF-α Lod lipopolysaccharide Fatty acid methyl ester limit of quantification limit of detection Nitric oxide pathway Nitric oxide Polysaccharides electron ionization

Related Topics

Physical Sciences and Engineering Chemistry Organic Chemistry

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GC-MS detection and quantification of lipopolysaccharides in polysaccharides through 3-O-acetyl fatty acid methyl esters

Authors

Arquimedes PaixÃ£o de Santana-Filho, Guilhermina Rodrigues Noleto, Philip Albert James Gorin, Lauro Mera de Souza, Marcello Iacomini, Guilherme Lanzi Sassaki,