Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10600044 | Carbohydrate Polymers | 2005 | 5 Pages |
Abstract
Specific enzymatic degradation in combination with chromatographic and spectrometric techniques was used to understand acetyl group distribution over the amylopectin populations of differently sized granule fractions from potato and sweet potato starches. The hydrolysates obtained after α-amylase, Ã-amylase, pullulanase, and the combination of pullulanase, α-amylase and amyloglucosidase treatment were investigated by high-performance size-exclusion chromatography (HPSEC), high-performance anion-exchange chromatography (HPAEC) and Maldi-Tof-MS (Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometry). The acetyl groups were found to be located near the branching point, in the external chain and in the internal chain regions. The acetyl group distributions were different for amylopectin from different granule size fractions. Higher DP (degree of polymerization) fragments were present in the digests of acetylated amylopectin populations of the small size granule starches. Our studies confirmed that acetyl groups were unevenly distributed over the amylopectin populations.
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Physical Sciences and Engineering
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Authors
Zhenghong Chen, Junrong Huang, Patricia Suurs, Henk A. Schols, Alphons G.J. Voragen,