Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10602103 | Carbohydrate Polymers | 2011 | 7 Pages |
Abstract
The self-assembly of rod-coil carboxymethyl konjac glucomannan-graft-poly(ethylene glycol) (CKGM-g-PEG) and α-cyclodextrin (α-CD) complexes were investigated and used as encapsulating hollow nanospheres for the enzyme glucose oxidase (GOX) in aqueous solution. These hollow nanospheres exhibited “cell-like” semi-permeability allowing enzyme substrates to pass through the surface while restricting the encapsulated enzyme (i.e. GOX) to the interior. Encapsulated GOX exhibited higher thermostability, optimal enzymatic activity over a wider pH range and improved storage stability in comparison to free un-encapsulated GOX. In addition, these CKGM-g-PEG/α-CD hollow nanospheres showed in vitro biocompatibility when exposed to L929 cells when tested using MTT viability assay. These studies suggested that self-assembly of CKGM-g-PEG and α-CD to form stable nanospheres may be an effective method for enzyme encapsulation with numerous biomedical applications.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Quan Li, Bing Xia, Mickey Branham, Wei Ha, Hao Wu, Shu-Lin Peng, Li-Sheng Ding, Bang-Jing Li, Sheng Zhang,