Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10603066 | Carbohydrate Polymers | 2011 | 7 Pages |
Abstract
In this research, a novel derivative of chitooligosaccharides (COS) was synthesized by covalently linking gallic acid and COS (gallate-COS) via carbodiimide to improve cellular antioxidant activity. The direct intracellular radical scavenging effect of gallate-COS by 2â²,7â²-dichlorofluorescin diacetate (DCFH-DA) method, and the inhibition of oxidation of cellular macromolecules, such as DNA, protein and lipid in mouse macrophages (RAW264.7 cells) were determined. Furthermore, with the treatment of gallate-COS, the expression levels of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione (GSH) were significantly increased according to reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. In addition, gallate-COS decreased reactive oxygen species induced activation of the nuclear transcription factor (NF-κB). Collectively, these results suggest that gallate-COS can be used as a potential natural compound-based antioxidant in the functional food and pharmaceutical industries.
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Dai-Hung Ngo, Zhong-Ji Qian, Thanh-Sang Vo, BoMi Ryu, Dai-Nghiep Ngo, Se-Kwon Kim,