Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10603097 | Carbohydrate Polymers | 2011 | 8 Pages |
Abstract
Chitosan oligomers show various biological activities. However, its molecular mechanisms remain unknown in LPS-stimulated macrophages. Here, we explored the inhibitive effects of chitosan oligomers on LPS-induced IL-6/TNF-α production in macrophages. The results indicated chitosan oligomers pretreatment effectively inhibited LPS-induced over-expression of both inflammatory cytokines. Signal transduction studies show chitosan oligomers may repress not only the phosphorylation of p38, ERK1/2, JNK, phosphatidylinositol 3-kinase (PI3K) and Akt, but also the activation of nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). Furthermore, both the activation of NF-κB/AP-1 and the subsequent IL-6/TNF-α over-expression in LPS-induced macrophages are inhibited by specific p38 inhibitor (SB203580), ERK1/2 inhibitor (PD98059), JNK inhibitor (SP600125) and PI3K inhibitor (LY294002). In conclusion, our investigation suggests chitosan oligomers inhibited the elevated expression of IL-6/TNF-α in LPS-induced macrophages, regulated by MAPKs and PI3K/Akt pathways dependent on NF-κB/AP-1 activation.
Keywords
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Pan Ma, Hong-Tao Liu, Peng Wei, Qing-Song Xu, Xue-Fang Bai, Yu-Guang Du, Chao Yu,