Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10603209 | Carbohydrate Polymers | 2005 | 6 Pages |
Abstract
Two kinds of water-insoluble polysaccharides (GM5-1 and GM6-1) were isolated from the mycelium of Ganoderma tsugae with 0.5 M NaOH aqueous solution at 25 and 65 °C successively. Their chemical structures were characterized by infrared spectroscopy, gas chromatography and 13C NMR. GM5-1 was composed of (1â3)-α-d-glucan and mannoxylan, while GM6-1 was mainly mannoxylan. The sample GM6-1 was fractionated into five fractions by nonsolvent addition, and their weight-average molecular mass (Mw) and intrinsic viscosity [η] were determined by laser light scattering (LLS), size-exclusion chromatography combined with multi-angle laser light scattering (SEC-LLS) and viscometer in 0.25 M LiCl/DMSO at 30 °C. The Mw dependences of [η] and the radius of gyration (ãS2ãz1/2) were found to be [η]=8.9Ã10â2Mw0.57 (cm3 gâ1) and ãS2ãz1/2=4.1Ã10â2Mw0.52 (nm) for GM6-1 in the Mw range from 1.50Ã106 to 4.81Ã106, implying a random coil conformations in the solution. Molecular parameters of GM6-1 were calculated on the basis of the wormlike cylinder chain to be 4.2 nm for persistent length (q) and 832 nmâ1 for molar mass per unit contour length (ML).
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Authors
Yanfei Peng, Lina Zhang, Yuying Zhang, Xiaojuan Xu, John F. Kennedy,