Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10603275 | Carbohydrate Polymers | 2013 | 9 Pages |
Abstract
Fucan is a term used to denominate sulfated l-fucose rich polysaccharides. Here, a heterofucan, named fucan B, was extracted from the Spatoglossum schröederi seaweed. This 21.5 kDa galactofucan inhibited CHO-K1 proliferation and migration when fibronectin was the substrate. Fucan B derivatives revealed that such effects depend on their degree of sulfation. Fucan B did not induce cell death, but promoted G1 cell cycle arrest. Western blotting and flow cytometry analysis suggest that fucan B binds to fibronectin and activates integrin, mainly integrin α5β1, which induces FAK/RAS/MEK/ERK activation. FAK activation inhibits CHO-K1 migration on fibronectin and ERK blocks cell cycle progression. This study indicates that fucan B could be applied in developing new antitumor drugs.
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Authors
Leonardo Thiago Duarte Barreto Nobre, Arthur Anthunes Jacome Vidal, Jailma Almeida-Lima, Ruth Medeiros Oliveira, Edgar Jean Paredes-Gamero, Valquiria Pereira Medeiros, Edvaldo Silva Trindade, Celia Regina Cavichiolo Franco, Helena Bonciani Nader,