Characterization and lectin microarray of an immunomodulatory heteroglucan from Pleurotus ostreatus mycelia

► Glucans isolated from P. ostreatus mycelia have a high mol. wt. of ∼2.7 × 106 Da. ► Glucose, mannose and fucose moieties with β and α linkage form its basic framework. ► Lectin array signify glucose, mannose and fucose residues in terminals and interior. ► The heteroglucan folded into a triple helical conformation. ► Antitumor activity was associated with immune cell activation on glucan treatment.