Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10604630 | Carbohydrate Polymers | 2011 | 9 Pages |
Abstract
Endothelial cell adhesion molecules have been recognized as an early step in inflammation and atherogenesis. The inhibition of TNF-α-induced expression of vascular cell adhesion molecule (VCAM-1) and intracellular cell adhesion molecule (ICAM-1) in human aortic endothelial cells (HAEC), following pretreatment with mixed-linkage (1 â 3), (1 â 4)-β-d-glucans (β-glucans) isolated from oat and barley, and the possible molecular structure-function relations, have been explored. The apparent molecular weight (Mw) of β-glucans varied in the ranges of 0.71-2.42 Ã 105 (barley) and 0.36-2.55 Ã 105 (oat); a higher ratio of 3-O-β-cellobiosyl-d-glucose to 3-O-β-cellotriosyl-d-glucose oligomers in the polymeric chains was also found for barley β-glucans. Analysis of variance showed that polysaccharide concentration, Mw and fine structure of β-glucans affected the VCAM-1 expression (P < 0.05); the reduction of VCAM-1 expression in stimulated HAEC was also dose-dependent (concentration range of 3.75-200 μg/ml β-glucans) and significant at polysaccharide levels > 6.25 μg/ml (P < 0.05). The inhibition of VCAM-1 expression was greater for barley β-glucans compared to oat, displaying a maximum anti-inflammatory activity at Mw â¼1.40 Ã 105. Instead, the expression of ICAM-1 was suppressed (P < 0.05) only at high polysaccharide concentrations (>100 μg/ml), with maximum activity at Mw â¼2.50 Ã 105. Structural differences between the oat and barley β-glucans did to seem to influence the ICAM-1 expression.
Keywords
EGMOBCIL-1βHBSICAM-1HPSECVCAM-1HEPESEBMintracellular cell adhesion moleculeFBShEGFDMEMPBSHPAEC3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideMTTPulsed amperometric detectorHAECinterleukin 1βcoronary artery diseaseDulbecco's minimal essential mediumdegree of polymerizationMolecular structurefetal bovine serumAortic endothelial cellshuman aortic endothelial cellsRefractive indexCADHuman epidermal growth factorHigh-performance anion-exchange chromatographyTNF-αphosphate buffer salineAnti-inflammatory activityendothelial growth mediumendothelial cell basal mediumcell adhesion moleculesvascular cell adhesion moleculeBarsMolecular weightPADhigh performance size exclusion chromatographyLDL-cholesterolgastro-intestinal
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Athina Lazaridou, Zoi Papoutsi, Costas G. Biliaderis, Paraskevi Moutsatsou,