Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10604686 | Carbohydrate Polymers | 2011 | 9 Pages |
Abstract
In this study, we examine the preparation of chitosan-coated magnetic nanoparticle (MNP) (chitosan-MNP) and the feasibility of using tissue plasminogen activator (tPA) covalently bound to chitosan-MNP surface (chitosan-MNP-tPA) for magnetic targeted delivery of the thrombolytic drug. The physicochemical properties of chitosan-MNP prepared with increasing chitosan/MNP ratios were characterized in detail. The optimum drug loading is reached when 0.5Â mg tPA is conjugated with 5Â mg chitosan-MNP where 95% drug is attached to the carrier with full retention of its thrombolytic activity. Under magnetic guidance, chitosan-MNP-tPA can reduce the blood clot lysis time by 58% compared with runs without magnetic targeting or by 53% compared with free tPA using the same drug dosage. Effective thrombolysis in response to chitosan-MNP-tPA under magnetic guidance is also demonstrated in a rat embolic model where one-fifth of the dose of tPA may exert similar thrombolytic efficacy of the drug.
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Authors
Jyh-Ping Chen, Pei-Chin Yang, Yunn-Hwa Ma, Tony Wu,