Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10605784 | Carbohydrate Research | 2010 | 9 Pages |
Abstract
MALDI-TOF mass spectrometry analyses of several oligosaccharides (aldoses) and oligosaccharide alditols derived from agaroses, kappa- and iota-carrageenans using different matrices (2,5-dihydroxybenzoic acid, nor-harmane, ferulic acid, and the ionic liquid matrices 2,5-dihydroxybenzoic acid-n-butylamine and ferulic acid-n-butylamine) were conducted. These carbohydrates were selected as model compounds to study the MALDI prompt and post-source decay (PSD) fragmentation processes of both families of oligosaccharides. Sulfated alditols showed in the negative-ion mode the molecular ion as [MâNa]â together with the species yielded by their prompt fragmentation (mainly desulfation) while the sulfated oligosaccharides (aldoses) showed mainly glycosidic prompt fragmentation (glycosidic C-cleavages and desulfation). Non-sulfated aldoses and alditols, which could only be analyzed in positive-ion mode ([M+Na]+), did not suffer any prompt fragmentation. The former yielded cross-ring fragmentation in the PSD mode. Best results were obtained by using 2,5-dihydroxybenzoic acid and/or nor-harmane as matrices for all the compounds studied.
Keywords
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
M.Kaniz Fatema, Hiroshi Nonami, Diogo R.B. Ducatti, Alan G. Gonçalves, M. Eugẻnia R. Duarte, Miguel D. Noseda, Alberto S. Cerezo, Rosa Erra-Balsells, MarÃa C. Matulewicz,