Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10609882 | European Polymer Journal | 2011 | 7 Pages |
Abstract
The dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) with glycidyl methacrylate (GMA) in a toluene/2-methylpropan-1-ol mixture in the presence of Fe3O4 nanoparticles coated with oleic acid, produced monodisperse magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-GMA)) microspheres. Oxirane groups of the microspheres were ammonolyzed and then functionalized with streptavidin using cyanuric chloride. The final product contained 0.67Â mg of streptavidin per gram of wet magnetic P(HEMA-GMA) microspheres. The microspheres were characterized by elemental analysis, scanning electron microscopy, IR, UV-VIS and atomic absorption spectroscopy. The streptavidin-modified magnetic P(HEMA-GMA) microspheres were used for immobilization of biotinylated DNA and subsequent selective isolation of target DNA from complex samples using DNA/DNA hybridisation. Based on the highly selective recognition of streptavidin with a biotin-labeled DNA probe, DNA sensor was constructed for magnetic separation of DNA from real samples.
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Authors
Daniel Horák, Alena Å panová, Jana TvrdÃková, Bohuslav Rittich,