Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10613536 | Journal of Controlled Release | 2005 | 10 Pages |
Abstract
Liposomes were prepared, with a 1:1 PC:Col molar ratio with or without DSPE-PEG, by a thin-film hydration. Ex-vivo experiments were performed, incubating prolidase loaded liposomes with cultured fibroblasts from PD patients and from controls, to determine the amount of active enzyme delivered to cells. Evaluation of liposomes toxicity on cultured skin fibroblasts showed that liposomes did not interfer with cellular growth. Results showed that all the active prolidase encapsulated in the liposomes was completely vehiculated inside fibroblasts after 6 days incubation. SEM analysis suggests that prolidase is vehiculated inside the cell through liposome endocytosis.
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Authors
Paola Perugini, Khaolé Hassan, Ida Genta, Tiziana Modena, Franca Pavanetto, Giuseppe Cetta, Chiara Zanone, Paolo Iadarola, Annalia Asti, Bice Conti,