Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10614718 | Materials Science and Engineering: C | 2012 | 5 Pages |
Abstract
In this study, a bilayer system composed of N-[3-trimethoxysilyl propyl]-ethylene diamine (TEDA) and protein-A on silicon wafer was prepared by a simple two-step procedure. Self-assembly deposition of TEDA at optimal conditions resulted in the formation of homogeneous self-assembled monolayers (SAMs) ~Â 2.3Â nm thick with the surface roughness ~Â 0.38Â nm. The height value of protein-A overlayer was found to be ~Â 3.5Â nm, which is within experimental error of the diameter of a single protein-A (3Â nm). Immunoglobulin G (IgG) molecules were then immobilized on the bilayer system by protein-A - IgG specific interactions. Using this very simple approach, the IgG layer was formed almost of a monomolecular layer for longer adsorption time (~Â 100Â min), and it was packed densely for adsorption time longer than 100Â min, which resulted in the increase of the amount of IgG immobilized. The use of a bilayer system composed of TEDA and protein A on silicon wafer opens the door for a fundamental understanding of how protein A affects IgG orientation on the surface and also indicates a useful guide to designing surfaces for applications such as immunosensors and biochips.
Keywords
Related Topics
Physical Sciences and Engineering
Materials Science
Biomaterials
Authors
Adem Zengin, Tuncer Caykara,