Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10614753 | Materials Science and Engineering: C | 2011 | 5 Pages |
Abstract
A novel potentiometric urea biosensor has been fabricated with urease (Urs) immobilized multi-walled carbon nanotubes (MWCNTs) embedded in silica matrix deposited on the surface of indium tin oxide (ITO) coated glass plate. The enzyme Urs was covalently linked with the exposed free -COOH groups of functionalized MWCNTs (F-MWCNTs), which are subsequently incorporated within the silica matrix by sol-gel method. The Urs/MWCNTs/SiO2/ITO composite modified electrode was characterized by Fourier transform infrared (FTIR) spectroscopy, thermal gravimetric analysis (TGA) and UV-visible spectroscopy. The morphologies and electrochemical performance of the modified Urs/MWCNTs/SiO2/ITO electrode have been investigated by scanning electron microscopy (SEM) and potentiometric method, respectively. The synergistic effect of silica matrix, F-MWCNTs and biocompatibility of Urs/MWCNTs/SiO2 made the biosensor to have the excellent electro catalytic activity and high stability. The resulting biosensor exhibits a good response performance to urea detection with a wide linear range from 2.18 Ã 10â 5 to 1.07 Ã 10â 3 M urea. The biosensor shows a short response time of 10-25 s and a high sensitivity of 23 mV/decade/cm2.
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Authors
Tarushee Ahuja, D. Kumar, Nahar Singh, A.M. Biradar, Rajesh Rajesh,