Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10693092 | Ultrasound in Medicine & Biology | 2005 | 6 Pages |
Abstract
We reported the use of high-frequency ultrasound biomicroscopy (UBM) in the quantitative analysis of early tumor growth in mice bearing melanoma xenografts in a noninvasive longitudinal assay. Initially, measurements of tumor width, depth and length were obtained using on-screen UBM calipers in real time and tumor volume was calculated with the standard ellipsoid formula w d l Ï/6. We were able to detect initiating minute tumor nodules, with the lower limit of detection at â 0.01 mm3 in volume. Successive parallel cross-sectional UBM images (33 μm step) encompassing the complete length of these tumors were also obtained and reconstructed into 3-D representations. Subsequent segmentational volumetric analysis provided a measure of tumor volume. Volume measurements using the two techniques were highly correlated when all 33 xenografts were studied (r = 0.9813, p < 0.0001) and a lower degree of correlation was measured with a subset of early small tumors (r = 0.7973, n = 16, p = 0.0004). Further analysis demonstrated that 3-D segmentational volumetric analysis yielded volume estimates that were often smaller than the caliper-and-formula calculation for most early developing xenografts. Thus, 3-D UBM imaging and segmentation is expected to be especially valuable for small tumors that were observed to grow in irregular shapes other than ellipsoids. (E-mail: acheung@sten.sunnybrook.utoronto.ca)
Keywords
Related Topics
Physical Sciences and Engineering
Physics and Astronomy
Acoustics and Ultrasonics
Authors
Alison M.Y. Cheung, Allison S. Brown, Lauren A. Hastie, Viviene Cucevic, Marcia Roy, James C. Lacefield, Aaron Fenster, F. Stuart Foster,