| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10737932 | Free Radical Biology and Medicine | 2012 | 9 Pages |
Abstract
⺠Ascorbate can enhance the activity of endothelial NO synthase (eNOS) within minutes. ⺠This effect occurs independent of stabilization of tetrahydrobiopterin. ⺠Ascorbate modulates eNOS phosphorylation in a PP2A- and AMPK-dependent manner.
Keywords
PI3Khemagglutinin tagHA-tagPhosphatidylinositol 3-KinasestetrahydrobiopterinBH4PKCeNOSHUVECPP2ATLCHEPESAMPKDMEMFBS4-(2-hydroxyethyl)-1-piperazineethanesulfonic acidAMP-activated protein kinaseDMSODulbecco's modified Eagle's mediumSDS–PAGEAscorbatesodium dodecyl sulfate–polyacrylamide gel electrophoresisDimethyl sulfoxideFree radicalsfetal bovine serumHuman umbilical vein endothelial cellendothelial nitric oxide synthaseHIVhuman immunodeficiency virusprotein phosphatase 2AProtein kinase CHPLChigh-performance liquid chromatographythin-layer chromatographyAMP-activated kinase
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Authors
Angela Ladurner, Christoph A. Schmitt, Daniel Schachner, Atanas G. Atanasov, Ernst R. Werner, Verena M. Dirsch, Elke H. Heiss,