Internalization and induction of antioxidant messages by microvesicles contribute to the antiapoptotic effects on human endothelial cells

Article ID	Journal	Published Year	Pages	File Type
10737953	Free Radical Biology and Medicine	2012	12 Pages	PDF

Abstract

âº Microvesicles (MVs) from T lymphocytes protect endothelial cells against apoptosis. âº Effect of MVs is linked with caspase inhibition and Bax and Bcl-2 expressions. âº Internalization of MVs induces antioxidant defense to endothelial cells. âº MVs activate the Sonic Hedgehog cascade, independently of mitochondrial reactive oxygen species.

Keywords

NOS EC(s)PHA mRNA Phorbol-12-myristate-13-acetate TBS HRP Microvesicles HUVECS ERK NADPH TCTP Phytohemagglutinin DETC CMH IGF-1 FGF-2 PI3-K TBS-T WST-1 EGF 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt cycl EC-SOD MnTMPyP FBS PBS BSA l-NA PMA MAPK Mn-SOD Nω-nitro-l-arginine messenger RNA ROS Hydrogen peroxide Adenosine Triphosphate ATP bovine serum albumin actinomycin D Tris-buffered saline EPR TUNEL Cytoprotection Apoptosis Shh diethyldithiocarbamate Electronic paramagnetic resonance fetal bovine serum endothelial cell(s)Human umbilical vein endothelial cells Endothelial cells extracellular superoxide dismutase manganese superoxide dismutase cyclopamine sonic hedgehog Insulin growth factor 1 epidermal growth factor fibroblast growth factor 2 Vascular Endothelial Growth Factor (VEGF)Vascular Endothelium Growth Factor Phosphatidylinositol 3-kinase Act D Phosphate-buffered saline copper/zinc superoxide dismutase Cu/Zn-SOD Nitric oxide nitric oxide synthase nicotinamide adenine dinucleotide phosphate-oxidase H2O2 Horseradish peroxidase Translationally controlled tumor protein mitogen-activated protein kinase Propidium iodide extracellular-signal-regulated kinase Reactive oxygen species