| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10737983 | Free Radical Biology and Medicine | 2012 | 8 Pages |
Abstract
⺠Sperm from males without major ASGs had higher levels of DNA-base adduct formation. ⺠DNA-damaged sperm can effect fertilization. ⺠The resulting embryos had abnormal levels of growth and DNA repair proteins. ⺠Zygotes and 2-cell embryos sired by males without major ASGs show high DNA damage. ⺠Activation of DNA repair was found in 2-cell embryos.
Keywords
p.c.GPX8OHdGCATzygotessDNAdouble strand DNA53BP1dsDNAP53-binding protein 1p53TALPMale accessory sex glandsPBS8-hydroxy-2′-deoxyguanosineasgBSASingle strand DNAROSHydrogen peroxideDNA damagebovine serum albuminsuperoxide anionsPolyunsaturated fatty acidsPUFApolar bodyRoom temperatureRad51Hydroxyl radicalsPhosphate-buffered salineHomologous recombinationsperm nucleusHamsterH2O2Catalaseglutathione peroxidaseReactive oxygen species
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Authors
Hong Chen, Su-Bin Liao, May P.L. Cheung, Pak Ham Chow, Annie L.M. Cheung, Wai Sum O,