| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10737986 | Free Radical Biology and Medicine | 2012 | 9 Pages |
Abstract
⺠PTI is the ratio between thiolated proteins and protein -SH groups in plasma. ⺠A spectrophotometric method to measure protein mixed disulfides for PTI is proposed. ⺠PTI was shown to be influenced by age and alkaptonuria.
Keywords
HGACysGSSGGSHN-ethylmaleimideTCADTNBcysteinylglycineHSADTTIAAHcysRSDdithiotreitolLLOQPTIP-SHmBrB5,5′-dithiobis-(2-nitrobenzoic acid)BSAROSγ-glutamylcysteinehuman serum albuminbovine serum albumintrichloroacetic acid.Homogentisic acidiodoacetic acidrelative standard deviationOxidative stresslow molecular mass thiolsCysglylower limit of quantificationrelative errorCysteineMonobromobimaneNEMhomocysteineProtein thiolsPlasmaGlutathioneglutathione disulfideReactive oxygen species
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Ageing
Authors
Daniela Giustarini, Isabella Dalle-Donne, Sauro Lorenzini, Enrico Selvi, Graziano Colombo, Aldo Milzani, Paolo Fanti, Ranieri Rossi,