| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10738141 | Free Radical Biology and Medicine | 2012 | 13 Pages |
Abstract
⺠Cigarette smoke increases protein carbonylation in human gingival fibroblasts (HGFs). ⺠The main carbonylated proteins were identified by means of redox proteomics. ⺠In HGFs, cigarette smoke decreased protein thiols and rapidly depleted GSH. ⺠GSH consumption is due to the export of GSH-acrolein and GSH-crotonaldehyde adducts
Keywords
DMEMmBrBHGFECLTPMDAPIDCFH-DAGAPDHN-ethylmaleimideTCAHSAFITCPVDFDcfDNPH2′,7′-dichlorodihydrofluorescein diacetate2′,7′-dichlorofluorescein2,4-dinitrophenylhydrazine4′,6-diamidino-2-phenylindoleDulbecco's modified Eagle's mediumROShuman serum albumintrichloroacetic acidenhanced chemiluminescenceCigarette smokepolyvinylidene difluoridetotal particulate matterFree radicalsfluorescein isothiocyanateHuman gingival fibroblastsMonobromobimaneNEMProtein thiolsRedox proteomicsProtein carbonylationglyceraldehyde-3-phosphate dehydrogenaseReactive oxygen species
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Authors
Graziano Colombo, Isabella Dalle-Donne, Marica Orioli, Daniela Giustarini, Ranieri Rossi, Marco Clerici, Luca Regazzoni, Giancarlo Aldini, Aldo Milzani, D. Allan Butterfield, Nicoletta Gagliano,