| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10738159 | Free Radical Biology and Medicine | 2012 | 10 Pages |
Abstract
⺠RhoA is essential for superoxide production by IgG-opsonized zymosan (IOZ) in macrophages. ⺠Rap1 also regulates superoxide generation by IOZ. ⺠RhoA and Rap1 rescue superoxide production in response to IOZ that were previously suppressedby Rap1 and RhoA defects, respectively. ⺠Rap1 activates Rac1, a component of the NADPH oxidase complex, via Vav2 GEF by IOZ. ⺠RhoA induces the release of Rac1 from Rac1-RhoGDI complex by competitive complex formation of phospho-RhoA-RhoGDI in response to IOZ.
Keywords
RBDRho-associated, coiled-coil containing protein kinasePBDRAP1GEFEpacPAKSOZRac1GDSpKaPMSFPBSERKRhoAFITCDMEMFBSGDIGSHGSTDulbecco's modified Eagle MediumMAPKlysophosphatidic acidNADPH oxidasedihydroethidiumfetal bovine serumSuperoxideGAPguanine nucleotide exchange factorPhagocytosisfluorescein isothiocyanatePhenylmethanesulfonyl fluoridePhosphate-buffered salineDHEExchange protein directly activated by cAMPGTPase activating proteinsprotein kinase Ap21-activated kinaseextracellular signal-regulated kinasesmitogen-activated protein kinasesGlutathioneglutathione S-transferaseRock
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Authors
Yi Li, Jae-Gyu Kim, Hee-Jun Kim, Mi-Young Moon, Jae-Yong Lee, Jaebong Kim, Sung-Chan Kim, Dong-Keun Song, Yong-Sun Kim, Jae-Bong Park,