Ankaflavin, a novel Nrf-2 activator for attenuating allergic airway inflammation

Article ID	Journal	Published Year	Pages	File Type
10738185	Free Radical Biology and Medicine	2012	9 Pages	PDF

Abstract

âº Ankaflavin (AK) is a yellow pigment derived from Monascus-fermented metabolites. âº AK ameliorates airway inflammation and mucus cell hyperplasia in lungs of mice. âº Inhibitory effect of AK on lung injury is abolished by siRNA for Nrf-2. âº AK elevates Nrf-2 translocation in vitro and in vivo.

Keywords

Th2 CAT ESI-MS GST ankaflavin GPX NRF-2 HO-1 TLC ICAM-1 NAC VCAM-1 OVA PAGE ECL RMR AREs Heme oxygenase-1 enhanced chemiluminescent NQO-1 T helper type 2 PVDF UDG PPAR-γ NF-κB Nuclear factor-erythroid 2 related factor 2 FBS SDS nuclear magnetic resonance keap1 DMSO Uracil DNA glycosylase H&E N-acetylcysteine ROS Antioxidant γ-aminobutyric acid periodic acid–Schiff Airway inflammation polyacrylamide gel electrophoresis Ovalbumin interleukin BALF NMR Oxidative stress tumor necrosis factor-α Dimethyl sulfoxide SOD sodium dodecyl sulfate fetal bovine serum Superoxide dismutase electrospray ionization–mass spectrometry antioxidant response elements TNF-α nuclear factor-κB Bronchoalveolar lavage fluid intercellular adhesion molecule-1 vascular cell adhesion molecule-1 PAS Hematoxylin and Eosin Kelch-like ECH-associated protein 1 Catalase high-performance liquid chromatography HPLC thin-layer chromatography GABA glutathione S-transferase glutathione peroxidase Reactive oxygen species peroxisome proliferator activated receptor-γ