| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10738186 | Free Radical Biology and Medicine | 2012 | 11 Pages |
Abstract
⺠Protein radicals are formed during cellular metabolism and in human disease. ⺠TEMPO nitroxide scavenges tyrosyl and tryptophanyl radicals with k 107-108 Mâ1 sâ1. ⺠Micromolar TEMPO concentrations prevent oxidation of cell proteins in macrophages. ⺠TEMPO protects cells from photosensitized death at attainable plasma concentrations. ⺠Protein radical scavenging may contribute to the beneficial effects of nitroxides.
Keywords
di-TyrSDSTyroLRETHBSSDOPAGSHTCA3,4-dihydroxyphenylalanineBSAbovine serum albumintrichloroacetic acidLong-range electron transferProtein oxidationElectron paramagnetic resonanceEPRPulse radiolysisFree radicalsTEMPOsodium dodecyl sulfatelactate dehydrogenaseLDHNitroxidesreduced glutathionehigh-pressure liquid chromatographyHPLC
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Authors
David I. Pattison, Magdalena Lam, Sujata S. Shinde, Robert F. Anderson, Michael J. Davies,