Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10738820 | Free Radical Biology and Medicine | 2005 | 9 Pages |
Abstract
The specificity of 4-amino-5-methylamino-2â²,7â²-difluorofluorescein (DAF-FM) for nitric oxide was evaluated in in vitro systems. The probe was found fairly specific for nitric oxide. Potential sources of artifacts include the autoxidation of DAF-FM, potentiated by light, and its oxidation by sources of superoxide and peroxyl radicals, leading to fluorescence spectra indistinguishable from those of the nitric oxide adduct. Although DAF-FM reacts with peroxynitrite, this reaction seems to be of secondary importance under quasi-physiological conditions. On the other hand, a simultaneous presence of a nitric oxide source and a superoxide or hydrogen peroxide decreases or increases the fluorescence of DAF-FM, respectively, resulting in biased estimates of nitric oxide production.
Keywords
ABAPS-nitroso-N-acetylpenicillamineONOO-SIN-12,2′-azobis(2-amidinopropane) hydrochlorideEcGSDAF-FMHBSS4-Amino-5-methylamino-2′,7′-difluorofluoresceinRNSHUVECSGSHDAF-FM-DADETA NONOateXanthine oxidase2′,7′-dichlorodihydrofluorescein diacetate4-Amino-5-Methylamino-2′,7′-Difluorofluorescein DiacetateH2DCFDAPMAROSHydrogen peroxideSODHuman umbilical vein endothelial cellsSuperoxideSuperoxide dismutasephorbol myristate acetateHank's buffered salt solutionendothelial cell growth supplementNitric oxidePeroxynitriteSNPGlutathionereactive nitrogen speciesReactive oxygen species
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Authors
Aneta Balcerczyk, MirosÅaw Soszynski, Grzegorz Bartosz,