Results of an international transferability study of the BINACLE (binding and cleavage) assay for inÂ vitro detection of tetanus toxicity

Article ID	Journal	Published Year	Pages	File Type
10852701	Biologicals	2014	6 Pages	PDF

Abstract

In conclusion, the data from this study indicate that the methodology of the BINACLE assay seems to be robust, reproducible and easily transferable between laboratories. These findings substantiate our notion that the method can be suitable for the routine testing of tetanus toxoids.

Keywords

PEI PBS IVI TMAO NIBSC TMB TeNT 3,3′,5,5′-tetramethylbenzidine BSA tris(2-carboxyethyl)phosphine hydrochloride bovine serum albumin tetanus neurotoxin Trimethylamine N-oxide Enzyme-linked immunosorbent assay ELISA TCEP Phosphate buffered saline Pipes National Institute for Biological Standards and Control

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry, Genetics and Molecular Biology (General)

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Results of an international transferability study of the BINACLE (binding and cleavage) assay for inÂ vitro detection of tetanus toxicity

Authors

Heike A. Behrensdorf-Nicol, Ursula Bonifas, Richard Isbrucker, Hanspeter Ottiger, Robert Tierney, Kay-Martin Hanschmann, Karin Weisser, Beate Krämer,