Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10877887 | Marine Genomics | 2015 | 8 Pages |
Abstract
Results showed that DSP gene expression had a clear and constant increase along the S. marinoi growth curve reaching its maximum during the senescent phase. On the contrary, PDCD4, DCD, TSG101 and MC did not show any significant variation. These findings indicate that the DSP gene is a possible PCD marker induced by aging in this diatom species. In contrast, levels of DSP transcripts induced by silica starvation were relatively low compared to those induced by cell aging suggesting differential activation and/or regulation of the PCD machinery in response to different stressful conditions. Our study also expands the list of reference genes available for the diatom S. marinoi for normalization of RT-qPCR data of cells cultivated under different growth phases or under silica starvation.
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Authors
Ida Orefice, Chiara Lauritano, Gabriele Procaccini, Adrianna Ianora, Giovanna Romano,