Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10879911 | Toxicon | 2012 | 8 Pages |
Abstract
⺠A system for heterologous expression of Conus ventricosus conotoxin Vn2 has been developed. ⺠Conotoxin Vn2 and its Asp2His mutant have been expressed fused to glutathione-S-transferase and purified to homogeneity. ⺠The toxicity of wild type and mutant conotoxins Vn2 has been assayed on the larvae of the moth Galleria mellonella. ⺠Conotoxin Vn2 has strong insecticidal properties at a dose of only 100 pmol/g of body weight. ⺠Mutation of Asp2 to His leads to enhanced toxicity in the larvae model system.
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Authors
Maria Carolina Spiezia, Cristiano Chiarabelli, Fabio Polticelli,