Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10880125 | Toxicon | 2011 | 6 Pages |
Abstract
Based on degradation of sphingomyelin/cholesterol liposomes containing entrapped horseradish peroxidase, we evaluated the Sphingomyelinase-D (SMase-D) activity of scorpion, spider and snake venoms by monitoring spectrophotometrically the product of oxidation of HRP released. The results indicate that Loxosceles crude venoms (Loxosceles intermedia, Loxosceles laeta, Loxosceles gaucho and Loxosceles similis) displayed SMase-D activity in a concentration-dependent manner. Furthermore, this activity was blocked by the anti-loxoscelic antivenom. However, Tityus serrulatus scorpion venom, Phoneutria nigriventer spider venom and Bothrops jararaca, Crotalus durissus, Lachesis muta and Micrurus frontalis snake venoms did not show measurable SMase-D activity.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry, Genetics and Molecular Biology (General)
Authors
Marco Túlio R. Gomes, Gabriela Guimarães, Frédéric Frézard, Evanguedes Kalapothakis, João Carlos Minozzo, Olga Meiri Chaim, Silvio Sanches Veiga, Sergio Costa Oliveira, Carlos Chávez-Olórtegui,